For this experiment, you will be given worms with a dominant mutation in the
rol-6 gene. You will also be given some wild
type worms so that you can observe the normal movement pattern.
You will then use RNAi (also known as RNA interference) to knockdown
expression of the mutant rol-6 allele. What do you expect will happen to the worms
after the RNAi treatment?
What is a wild type or a mutant? How do wild type and mutant develop similarly or differently? This module includes movies of wild type and 5 mutant C. elegans' early embryonic development. These movies are in DIC (Differential Interference Contrast) format with magnification about 100X.
Adult male, protruding
Same as previous, with different focal plane highlighted
At left: L3 male with slightly less developed tail. The animal on the right is L4 male with more developed tail.
Male animal: Fan, hook, half of the body
Staining transgenic worms for ß-galactosidase. Also includes a list of supplies and solutions required for ß-galactosidase staining lab.
Lab for observing bacterial-mediated feeding RNAi. Also includes solutions for use with bacterial-mediated feeding RNAi (as well as other labs).