Genetics

C. elegans for the Masses

Contributor: 
Eric D. Brenner, Antonio R. Herrera, Dave H. Fitch
Your email: 
eb50@nyu.edu

Useful methods for producing high quality cultures and movies for C. elegans teaching labs at large (and small) institutions.

RNAi lab with rol-6

Contributor: 
Lynn Boyd
Your email: 
boydl@uah.edu

For this experiment, you will be given worms with a dominant mutation in the
rol-6 gene. You will also be given some wild
type worms so that you can observe the normal movement pattern.
You will then use RNAi (also known as RNA interference) to knockdown
expression of the mutant rol-6 allele. What do you expect will happen to the worms
after the RNAi treatment?

Laboratory in Genetics

Contributor: 
Dr. E. Jane Hubbard

This is a project-based laboratory activity. Students will be engaged in activities of "forward genetics" and "functional genomics" (utilizing RNAi). For detailed lab procedure, please contact Dr. jhubbard [at] saturn [dot] med [dot] nyu [dot] edu (E. Jane Hubbard) at New York University.

TCU genetics lab manual

Contributor: 
Phil Hartman and Misti Caudle

Genetics lab manual using C. elegans and E. coli.

Worm Breeding for Super Geniuses

Contributor: 
David S. Fay et. al.

A guide to genetic mapping in C. elegans.

Inhibition of unc-22 function using dsRNA

Contributor: 
Kirsten Crossgrove

DsRNAi by feeding lab for sophomore level genetics course using unc-22 gene. Handout provided to students (also includes instructions for online prelab). Includes prep sheet for dsRNAi by feeding lab.

Dpy-5 lab

Contributor: 
Lynn Boyd

Identification of dumpy worms; design of primers to amplify dpy-5 gene. Preparation of single worms for PCR, PCR reactions, gel electrophoresis.

Dice & Slice

Contributor: 
Brian Kahn

A 6-day hands-on inquiry unit. Activities include C. elegans phenotype observation, worm transfer, RNA interference, PCR and DNA electrophoresis. In addition, students have the opportunity to apply online tool BLAST to find out the gene sequence of the dsRNA underlying the interference of RNA (RNAi).

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