All Instructional Materials

Mutant Phenotype Analysis

Dominika Bienkowska

What is a wild type or a mutant? How do wild type and mutant develop similarly or differently? This module includes movies of wild type and 5 mutant C. elegans' early embryonic development. These movies are in DIC (Differential Interference Contrast) format with magnification about 100X.

Olfactory chemotaxis and adaptation

Noelle L'Etoile
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Movies of 2X sped up C. elegans as they chemotax toward a point source of 10-2 benzaldehyde. The corresponding clip of worms after exposure to benzaldehyde (10-4) for 80 minutes in the absence of food. 

RNAi lab with rol-6

Lynn Boyd
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For this experiment, you will be given worms with a dominant mutation in the
rol-6 gene. You will also be given some wild
type worms so that you can observe the normal movement pattern.
You will then use RNAi (also known as RNA interference) to knockdown
expression of the mutant rol-6 allele. What do you expect will happen to the worms
after the RNAi treatment?

RNAi protocols with solutions

Daryl Hurd

Lab for observing bacterial-mediated feeding RNAi. Also includes solutions for use with bacterial-mediated feeding RNAi (as well as other labs).

Serial Block Face Imaging of C. elegans with 3View

Joel Mancuso

Serial Block Face Imaging in the SEM, scans serial images of a resin embedded TEM like sample in the SEM.  By placing an ultramicrotome inside the chamber of a SEM, 50nm is removed from the surfaced, and the blockface is rescanned. This process is automated, and produces perfectly aligned serial images. This data set contains approximately 300 serial images that were acquired in 5 hours. Sample courtesy of Shigeki Watanabe, University of Utah.

TCU genetics lab manual

Phil Hartman and Misti Caudle

Genetics lab manual using C. elegans and E. coli.

Using Digital Microscopy

Madelaine Travaille and Sandra D. Adams

Travaille, M. & Adams, S. (2006). Using digital microscopy. The Science Teacher. 73(4), 50-54.

In this article, video digital microscopy was used that allowed students to capture images of C. elegans [both time-laped and still] to study animal behavior and development.

Note: you must be an NSTA member to access the article online.

Worm beta-gel lab

Lynn Boyd

Staining transgenic worms for ß-galactosidase. Also includes a list of supplies and solutions required for ß-galactosidase staining lab.

Worm Breeding for Super Geniuses

David S. Fay et. al.

A guide to genetic mapping in C. elegans.