Multiphoton Fluorescence Excitation Microscopy

Multiphoton microscopy is used to generate high resolution optical sections to image fluorescent signals, usually in live specimens.

This is a C. elegans embryo in the first mitosis stage (anterior at the left and posterior at the right). Microtubules (MTs) are labeled using GFP.

MPFE Compared to Laser Scanning Confocal Microscopy

By using multi-photon for fluorophore excitation:

(Enable "selective excitation", 31 KB, movie length: 1' 7")

(Enable "deeper sectioning & better specimen viability", 1 MB, movie length: 48")

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MPFEDeeper.swf1005.18 KB
MPFESelective.swf30.72 KB