Spliced Genome

  • Combine the information here with the Protein Info page might provide some interesting insights. For example, the length of the genome or amino acid sequence or the place where the sequence difference occurs between wild-type and mutants.
  • By combining the information here with the Protein Info page, you can also compare the DNA codon to the amino acid sequence, some interesting relationship might be found.
  • Remember the characteristics you did while first saw the wild type and mutants? Try to think how the info provided in pages like Protein Info, Spliced Genome and Unspliced Genome can be used to explain those characteristics differences.
  • Tools for sequences comparison are provided down in the bottom of this page. For each program, you can simply copy and paste the sequence of your interest to any of the programs and see how sequences differ from each other. See whether you can gain some insight on the difference.
  • You might find DOTLET's alignment tool helpful while comparing spliced and unspliced DNA sequences.
Try to combine the info here with the "Unspliced Genome Inf", interesting perspectives might be found, such as the length of the sequences, and the number of codons.
Genotype and Relative Info
Movie Subject
Genotype
Length of the DNA Sequence (base pair)
Number of Codons of This Gene
WT
wild-type
2121 bps
707
mutant 1
zyg-1(oj7)
2121 bps
707
mutant 2
zyg-1(b1)
2121 bps
707
mutant 3
zyg-1(it4)
1848 bps
616
mutant 4
zyg-1(it37)
2055 bps
685

 

Nucleotide Sequence
Movie Subject
Nucleotide (DNA) sequence of alleles of the zyg-1 Gene
WT
atgagcggtg ggaagagtgg ttcaagattg agtgcctatt cacatttaaa agaaatcggg aaaggtggat tcggcgttgt atatgcagca caaagagaaa atggagagaa ggtggcgatt aagaggatcg ataaaaaggt gccaaaaaat cgtgtttgga cggaaattca aacgatgaag gaattgaaga aaagcaaata tgtggtcgag ttttacgaag atttcgttga agatggttat acatacatag tcatggagtt gtgcgaagga ggatctcttc aagcttatgt gcgggagcac ggagctctgg acgacgcaac agcagttcat gttttacgac agctgatttc tgctgtatct tttatgcaca gggtgaatgt tattcaccga gacctctcgg ctggtaatgt gttcatcaaa gactcgaaaa agaagaaaat gacggttaaa cttggcgatt ttggcctcgc gaccactctt gggcgtggtg agaccacatg tacaattgtc ggaacaccag gattcattgc tccacaagta tacgatcaag aatataccca gtctgccgat gtatattctc ttggtgcagt tctctatact atgctcactg ctcgtaatcc cccacctaaa ggactaccac caacctgcgg aatgtcacca aatgctgcta gattagtgga acaaatgatg gatacagatg caaagaagcg tattccgtta acgcagatag ttttatcaga gttcatgtac gagaatacga atgaaaatgc ggtaatcttc tcgagagaac actcgcggga tggacgacga cagagatcga gggaaccagt aagatcctca agagatgatc gatctcgaga tggcagagct ctgataaggt cttcgagtca acctgcacat tctggcagag caccgctgag caatcgacca attcatgaca ggatgccttc tacatcttca agaggatttg attctgagag aggaagagaa cgggatagag acagtggccg cggtaccgta cctccaagcc gtgaagatcg aaaccgttca cagttgtggc cgatccgaat ggataggtta gaaggacaaa gagtttgtac tgctggagga cgatatattg ttgaattgga tactcgttgt cggtttgaag ttgcagctca agggaatttc gttaaacgaa ttttgattgt cgaagttgat gaaatggttc aaacggttta tgtacatcga atacctgata gaacagtacg cggaagaaat ggagaagaag aattgattac gttgacgaac aatccttttg tttatacttc atattcccaa atgccaaaag aagtccaaaa cgattatatg cgtctccaaa agatggttgc tgtgacgata agtggtcgag tagcaaaagt aacatttcga cgtccatcac aattcccaga tgctcaagct caacttatgg agaatggaga tctccgtatc aaacttccga gatcagtaat tgttcgtaaa atggataatg gtgaaatttt caattgcatc gatggaatcg cgacccaaaa acaggcagtt tctggaataa cacttacaaa agtgaatgaa gtatataaat atctaataag attcgagcaa tgtcttaacg gaatggatcg aggaatggtg tgctttccca ttgttttcag tgcaggaact aatatggttg gcagttcgcc gtcttcatta atgccatcag gatcttctca aacttcccga tttccattta gtaatttatc gaacaatcag ccatcattag ttccccattc ggctcctttt cttacgaaac caacttcatc tcaacgagca tcatcggcta atgttcagag aagggtatca actgatgaaa attcgtcacc tagtgtggct ccatcaaagt ataaaataaa aattgaccct acaactcaaa aagttcgatc aattcaagca acagacggtc gtgtgctacg atgttccaca agtaaagcgg atcaattcat tttcacagat ccggcgattc gtcctgatga tcaacgattt atgcgaactg atcgagtacc tgacagagct tcagaaatgc ttcatacgct gtgcgaacgt atgagaaaac tacatcaata a
Mutant 1
atgagcggtg ggaagagtgg ttcaagattg agtgcctatt cacatttaaa agaaatcggg aaaggtggat tcggcgttgt atatgcagca caaagagaaa atggagagaa ggtggcgatt aagaggatcg ataaaaaggt gccaaaaaat cgtgtttgga cggaaattca aacgatgaag gaattgaaga aaagcaaata tgtggtcgag ttttacgaag atttcgttga agatggttat acatacatag tcatggagtt gtgcgaagga ggatctcttc aagcttatgt gcgggagcac ggagctctgg acgacgcaac agcagttcat gttttacgac agctgatttc tgctgtatct tttatgcaca gggtgaatgt tattcaccga gacctctcgg ctggtaatgt gttcatcaaa gactcgaaaa agaagaaaat gacggttaaa cttggcgatt ttggcctcgc gaccactctt gggcgtggtg agaccacatg tacaattgtc ggaacaccag gattcattgc tccacaagta tacgatcaag aatataccca gtctgccgat gtatattctc ttggtgcagt tctctatact atgctcactg ctcgtaatcc cccacctaaa ggactaccac caacctgcgg aatgtcacca aatgctgcta gattagtgga acaaatgatg gatacagatg caaagaagcg tattccgtta acgcagatag ttttatcaga gttcatgtac gagaatacga atgaaaatgc ggtaatcttc tcgagagaac actcgcggga tggacgacga cagagatcga gggaaccagt aagatcctca agagatgatc gatctcgaga tggcagagct ctgataaggt cttcgagtca acctgcacat tctggcagag caccgctgag caatcgacca attcatgaca ggatgccttc tacatcttca agaggatttg attctgagag aggaagagaa cgggatagag acagtggccg cggtaccgta cctccaagcc gtgaagatcg aaaccgttca cagttgtggc cgatccgaat ggataggtta gaaggacaaa gagtttgtac tgctggagga caatatattg ttgaattgga tactcgttgt cggtttgaag ttgcagctca agggaatttc gttaaacgaa ttttgattgt cgaagttgat gaaatggttc aaacggttta tgtacatcga atacctgata gaacagtacg cggaagaaat ggagaagaag aattgattac gttgacgaac aatccttttg tttatacttc atattcccaa atgccaaaag aagtccaaaa cgattatatg cgtctccaaa agatggttgc tgtgacgata agtggtcgag tagcaaaagt aacatttcga cgtccatcac aattcccaga tgctcaagct caacttatgg agaatggaga tctccgtatc aaacttccga gatcagtaat tgttcgtaaa atggataatg gtgaaatttt caattgcatc gatggaatcg cgacccaaaa acaggcagtt tctggaataa cacttacaaa agtgaatgaa gtatataaat atctaataag attcgagcaa tgtcttaacg gaatggatcg aggaatggtg tgctttccca ttgttttcag tgcaggaact aatatggttg gcagttcgcc gtcttcatta atgccatcag gatcttctca aacttcccga tttccattta gtaatttatc gaacaatcag ccatcattag ttccccattc ggctcctttt cttacgaaac caacttcatc tcaacgagca tcatcggcta atgttcagag aagggtatca actgatgaaa attcgtcacc tagtgtggct ccatcaaagt ataaaataaa aattgaccct acaactcaaa aagttcgatc aattcaagca acagacggtc gtgtgctacg atgttccaca agtaaagcgg atcaattcat tttcacagat ccggcgattc gtcctgatga tcaacgattt atgcgaactg atcgagtacc tgacagagct tcagaaatgc ttcatacgct gtgcgaacgt atgagaaaac tacatcaata a
Mutant 2
atgagcggtg ggaagagtgg ttcaagattg agtgcctatt cacatttaaa agaaatcggg aaaggtggat tcggcgttgt atatgcagca caaagagaaa atggagagaa ggtggcgatt aagaggatcg ataaaaaggt gccaaaaaat cgtgtttgga cggaaattca aacgatgaag gaattgaaga aaagcaaata tgtggtcgag ttttacgaag atttcgttga agatggttat acatacatag tcatggagtt gtgcgaagga ggatctcttc aagcttatgt gcgggagcac ggagctctgg acgacgcaac agcagttcat gttttacgac agctgatttc tgctgtatct tttatgcaca gggtgaatgt tattcaccga gacctctcgg ctggtaatgt gttcatcaaa gactcgaaaa agaagaaaat gacggttaaa cttggcgatt ttggcctcgc gaccactctt gggcgtggtg agaccacatg tacaattgtc ggaacaccag gattcattgc tccacaagta tacgatcaag aatataccca gtctgccgat gtatattctc ttggtgcagt tctctatact atgctcactg ctcgtaatcc cccacctaaa ggactaccac caacctgcgg aatgtcacca aatgctgcta gattagtgga acaaatgatg gatacagatg caaagaagcg tattccgtta acgcagatag ttttatcaga gttcatgtac gagaatacga atgaaaatgc ggtaatcttc tcgagagaac actcgcggga tggacgacga cagagatcga gggaaccagt aagatcctca agagatgatc gatctcgaga tggcagagct ctgataaggt cttcgagtca acctgcacat tctggcagag caccgctgag caatcgacca attcatgaca ggatgccttc tacatcttca agaggatttg attctgagag aggaagagaa cgggatagag acagtggccg cggtaccgta cctccaagcc gtgaagatcg aaaccgttca cagttgtggc cgatccgaat ggataggtta gaaggacaaa gagtttgtac tgctggagga cgatatattg ttgaattgga tactcgttgt cggtttgaag ttgcagctca agggaatttc gttaaacgaa ttttgattgt cgaagttgat gaaatggttc aaacggttta tgtacatcga atacctgata gaacagtacg cggaagaaat ggagaagaag aattgattac gttgacgaac aatctttttg tttatacttc atattcccaa atgccaaaag aagtccaaaa cgattatatg cgtctccaaa agatggttgc tgtgacgata agtggtcgag tagcaaaagt aacatttcga cgtccatcac aattcccaga tgctcaagct caacttatgg agaatggaga tctccgtatc aaacttccga gatcagtaat tgttcgtaaa atggataatg gtgaaatttt caattgcatc gatggaatcg cgacccaaaa acaggcagtt tctggaataa cacttacaaa agtgaatgaa gtatataaat atctaataag attcgagcaa tgtcttaacg gaatggatcg aggaatggtg tgctttccca ttgttttcag tgcaggaact aatatggttg gcagttcgcc gtcttcatta atgccatcag gatcttctca aacttcccga tttccattta gtaatttatc gaacaatcag ccatcattag ttccccattc ggctcctttt cttacgaaac caacttcatc tcaacgagca tcatcggcta atgttcagag aagggtatca actgatgaaa attcgtcacc tagtgtggct ccatcaaagt ataaaataaa aattgaccct acaactcaaa aagttcgatc aattcaagca acagacggtc gtgtgctacg atgttccaca agtaaagcgg atcaattcat tttcacagat ccggcgattc gtcctgatga tcaacgattt atgcgaactg atcgagtacc tgacagagct tcagaaatgc ttcatacgct gtgcgaacgt atgagaaaac tacatcaata a
Mutant 3
atgagcggtg ggaagagtgg ttcaagattg agtgcctatt cacatttaaa agaaatcggg aaaggtggat tcggcgttgt atatgcagca caaagagaaa atggagagaa ggtggcgatt aagaggatcg ataaaaaggt gccaaaaaat cgtgtttgga cggaaattca aacgatgaag gaattgaaga aaagcaaata tgtggtcgag ttttacgaag atttcgttga agatggttat acatacatag tcatggagtt gtgcgaagga ggatctcttc aagcttatgt gcgggagcac ggagctctgg acgacgcaac agcagttcat gttttacgac agctgatttc tgctgtatct tttatgcaca gggtgaatgt tattcaccga gacctctcgg ctggtaatgt gttcatcaaa gactcgaaaa agaagaaaat gacggttaaa cttggcgatt ttggcctcgc gaccactctt gggcgtggtg agaccacatg tacaattgtc ggaacaccag gattcattgc tccacaagta tacgatcaag aatataccca gtctgccgat gtatattctc ttggtgcagt tctctatact atgctcactg ctcgtaatcc cccacctaaa ggactaccac caacctgcgg aatgtcacca aatgctgcta gattagtgga acaaatgatg gatacagatg caaagaagcg tattccgtta acgcagatag ttttatcaga gttcatgtac gagaatacga atgaaaatgc ggtaatcttc tcgagagaac actcgcggga tggacgacga cagagatcga gggaaccagt aagatcctca agagatgatc gatctcgaga tggcagagct ctgataaggt cttcgagtca acctgcacat tctggcagag caccgctgag caatcgacca attcatgaca ggatgccttc tacatcttca agaggatttg attctgagag aggaagagaa cgggatagag acagtggccg cggtaccgta cctccaagcc gtgaagatcg aaaccgttca cagttgtggc cgatccgaat ggataggtta gaaggacaaa gagtttgtac tgctggagga cgatatattg ttgaattgga tactcgttgt cggtttgaag ttgcagctca agggaatttc gttaaacgaa ttttgattgt cgaagttgat gaaatggttc aaacggttta tgtacatcga atacctgata gaacagtacg cggaagaaat ggagaagaag aattgattac gttgacgaac aatccttttg tttatacttc atattcccaa atgccaaaag aagtccaaaa cgattatatg cgtctccaaa agatggttgc tgtgacgata agtggtcgag tagcaaaagt aacatttcga cgtccatcac aattcccaga tgctcaagct caacttatgg agaatggaga tctccgtatc aaacttccga gatcagtaat tgttcgtaaa atggataatg gtgaaatttt caattgcatc gatggaatcg cgacccaaaa acaggcagtt tctggaataa cacttacaaa agtgaatgaa gtatataaat atctaataag attcgagcaa tgtcttaacg gaatggatcg aggaatggtg tgctttccca ttgttttcag tgcaggaact aatatggttg gcagttcgcc gtcttcatta atgccatcag gatcttctca aacttcccga tttccattta gtaatttatc gaacaatcag ccatcattag ttccccattc ggctcctttt cttacgaaac caacttcatc tcaacgagca tcatcggcta atgtttag
Mutant 4
atgagcggtg ggaagagtgg ttcaagattg agtgcctatt cacatttaaa agaaatcggg aaaggtggat tcggcgttgt atatgcagca caaagagaaa atggagagaa ggtggcgatt aagaggatcg ataaaaaggt gccaaaaaat cgtgtttgga cggaaattca aacgatgaag gaattgaaga aaagcaaata tgtggtcgag ttttacgaag atttcgttga agatggttat acatacatag tcatggagtt gtgcgaagga ggatctcttc aagcttatgt gcgggagcac ggagctctgg acgacgcaac agcagttcat gttttacgac agctgatttc tgctgtatct tttatgcaca gggtgaatgt tattcaccga gacctctcgg ctggtaatgt gttcatcaaa gactcgaaaa agaagaaaat gacggttaaa cttggcgatt ttggcctcgc gaccactctt gggcgtggtg agaccacatg tacaattgtc ggaacaccag gattcattgc tccacaagta tacgatcaag aatataccca gtctgccgat gtatattctc ttggtgcagt tctctatact atgctcactg ctcgtaatcc cccacctaaa ggactaccac caacctgcgg aatgtcacca aatgctgcta gattagtgga acaaatgatg gatacagatg caaagaagcg tattccgtta acgcagatag ttttatcaga gttcatgtac gagaatacga atgaaaatgc ggtaatcttc tcgagagaac actcgcggga tggacgacga cagagatcga gggaaccagt aagatcctca agagatgatc gatctcgaga tggcagagct ctgataaggt cttcgagtca acctgcacat tctggcagag caccgctgag caatcgacca attcatgaca ggatgccttc tacatcttca agaggatttg attctgagag aggaagagaa cgggatagag acagtggccg cggtaccgta cctccaagcc gtgaagatcg aaaccgttca cagttgtggc cgatccgaat ggataggtta gaaggacaaa gagtttgtac tgctggagga cgatatattg ttgaattgga tactcgttgt cggtttgaag ttgcagctca agggaatttc gttaaacgaa ttttgattgt cgaagttgat gaaatggttc aaacggttta tgtacatcga atacctgata gaacagtacg cggaagaaat ggagaagaag aattgattac gttgacgaac aatccttttg tttatacttc atattcccaa atgccaaaag aagtccaaaa cgattatatg cgtctccaaa agatggttgc tgtgacgata agtggtcgag tagcaaaagt aacatttcga cgtccatcac aattcccaga tgctcaagct caacttatgg agaatggaga tctccgtatc aaacttccga gatcagtaat tgttcgtaaa atggataatg gtgaaatttt caattgcatc gatggaatcg cgacccaaaa acaggcagtt tctggaataa cacttacaaa agtgaatgaa gtatataaat atctaataag attcgagcaa tgtcttaacg gaatggatcg aggaatggtg tgctttccca ttgttttcag tgcaggaact aatatggttg gcagttcgcc gtcttcatta atgccatcag gatcttctca aacttcccga tttccattta gtaatttatc gaacaatcag ccatcattag ttccccattc ggctcctttt cttacgaaac caacttcatc tcaacgagca tcatcggcta atgttcagag aagggtatca actgatgaaa attcgtcacc tagtgtggct ccatcaaagt ataaaataaa aattgaccct acaactcaaa aagttcgatc aattcaagca acagacggtc gtgtgctacg atgttccaca agtaaagcgg atcaattcat tttcacagat ccggcgattc gtcctgatga tcaacgattt atgcgaactg attga

* Wondering where the wild type sequence is from? Try WormBase. Brief instructions on how to search can be found here.

Tools
Name of the program
Brief description of each tool

LALIGN

  •  Be able to compare two protein sequences or DNA sequences.
  •  Will show the percentage of identity between two sequences.
  • Be able to compare two protein or DNA sequences.
  • Also show the percentage of identity between two sequences.
  • Be able to compare two protein or DNA sequences. Also, some advanced function like comparing DNA to protein sequences.
  • With graphics to show the identity and alignment between sequences.

Note: For PC users only.

Brief instructions to get started using these programs.