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One of the most prevalent types of microscopy is optical sectioning microscopy (including phase, nomarski and confocal microscopy), where the researcher can make 4-dimensional recordings (3D + time) of their biological samples. A diagram of this type of system can be seen in the figure below. |
Basic 4D Digital Microscopy Setup
Image Credits:
1. DeVries, J.H., Thomas, C., White, J. (1996). Four-Dimensional Imaging: Computer Visualization of 3D Movements in Living Specimens. Science, 273, 603-607.