Confocal microscopy is used for imaging fluorescent signals, usually in fixed specimens. It generates high resolution optical sections.
This is a C. elegans embryo
at the 2-cell stage (bigger AB cell at bottom, P1 at the top). Red represents the membrane
, green represents the microtubules (MTs) and blue shows its chromosome
. This image combines images from 3 different channels. The membrane, MTs and chromosome were imaged using 3 channels, color-mapped to 3 colors (red, green, and blue).
By applying pinhole aperature to LSCM:
(Eliminate out-of-focus flare, 90 KB, movie length: 52″)
(The thickness of optical section is controllable, 160 KB, movie length: 15″)