Genetics Instructional Materials for college
LECTURE
LAB
dpy-5 lab (70
KB
): Identification
of dumpy worms; design of primers to amplify dpy-5 gene.
Worm PCR lab
(10 KB
):
Preparation of single worms for PCR, PCR reactions, gel electrophoresis.
~ by Lynn Boyd at University of Alabama in Huntsville
Worm beta-gal
lab (190 KB
):
Staining transgenic worms for ß-galactosidase.
Supplies
needed for worm (10 KB
):
Supplies and solutions for
ß-galactosidase staining lab.
~ by Lynn Boyd at University of Alabama in Huntsville
Inhibition
of unc-22 function using dsRNA – student handout (19
KB
): dsRNAi
by feeding lab for sophomore level genetics course using unc-22
gene. Handout provided to students (also includes instructions for
online prelab).
Inhibition
of unc-22 function using dsRNA – prep sheet (15 KB
): prep sheet for dsRNAi by feeding lab.
~ by Kirsten Crossgrove at University of Wisconsin – Whitewater
Worm
breeding for super geniuses (
):
a guide to genetic mapping in C. elegans.
~ by David S. Fay et. al.
TCU Genetics
lab manual (1.5 MB
):
Genetics lab manual using C. elegans and E. coli.
~ by Phil Hartman/Misti Caudle at Texas Christian University
Laboratory in Genetics: Description & Introduction (13 KB & 11 KB
): This is a project-based laboratory activity. Students will be engaged in activities of "forward genetics" and "functional genomics" (utilizing RNAi). For detailed lab procedure, please contact Dr. E. Jane Hubbard at New York University.
RNAi
protocols with tips (10 KB
):
Bacterial-mediated feeding RNAi.
Lab 1 solutions
(10 KB
):
Solutions for bacterial-mediated feeding RNAi (as well as other labs).
~ by Daryl Hurd at St. John Fisher College
Worm
Starter Kit (
):
The
worm goes to school introduces this kit that includes basic information
about manipulating C. elegans, microscopy and worm genetics.
~ by Institute of molecular and cell biology and national institute
of education, Singapore.
LECTURE & LAB
Genotype
v.s Phenotype module (hosted at LOCI): apply research resources
including C. elegans movies, images, DNA, protein data to help students
understand the relationship between genotype and phenotype.
Worm Educators:
Please contact
us to contribute your materials.