All Instructional Materials

BABEC C. elegans Labs

Katy Korsmeyer

This is a direct link to BABEC’s C. elegans Labs page. There are modules of hands-on activities, each with a student and teacher guide version. Current modules include “Meet the worms!”, “Synchronization”, “chemotaxis assay”, and “The Effect of Alcohol on Worms “.

C. elegans Early Embryo Development

Tim Walston

This PowerPoint lecture describes C. elegans early embryo development. It also includes detailed description about C. elegans gastrulation.

C. elegans for the Masses

Eric D. Brenner, Antonio R. Herrera, Dave H. Fitch
Your email: 
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Useful methods for producing high quality cultures and movies for C. elegans teaching labs at large (and small) institutions.

C. elegans Structures: high (20X60) magnification

Josh Bembenek

Outline of male tail fan

Adult male, protruding

Same as previous, with different focal plane highlighted

At left: L3 male with slightly less developed tail. The animal on the right is L4 male with more developed tail.

Shows fan, hook

Male animal: Fan, hook, half of the body

Cell Lineage

Fong-Mei Lu

The embryonic cell lineage of C. elegans from zygote to hatched larva provides an excellent tool for learning fundamental principals in developmental biology.

Dice & Slice

Brian Kahn

A 6-day hands-on inquiry unit. Activities include C. elegans phenotype observation, worm transfer, RNA interference, PCR and DNA electrophoresis. In addition, students have the opportunity to apply online tool BLAST to find out the gene sequence of the dsRNA underlying the interference of RNA (RNAi).

Dpy-5 lab

Lynn Boyd

Identification of dumpy worms; design of primers to amplify dpy-5 gene. Preparation of single worms for PCR, PCR reactions, gel electrophoresis.

Inhibition of unc-22 function using dsRNA

Kirsten Crossgrove

DsRNAi by feeding lab for sophomore level genetics course using unc-22 gene. Handout provided to students (also includes instructions for online prelab). Includes prep sheet for dsRNAi by feeding lab.

Laboratory in Genetics

Dr. E. Jane Hubbard

This is a project-based laboratory activity. Students will be engaged in activities of “forward genetics” and “functional genomics” (utilizing RNAi). For detailed lab procedure, please contact Dr. jhubbard [at] saturn [dot] med [dot] nyu [dot] edu (E. Jane Hubbard) at New York University.

Malthus Under a Microscope

Melinda M. Mueller

Mueller, M. (2007). Malthus Under a Microscope: Using the soil nematode Caenorhabditis elegans to test Darwin’s premises about populations. The American Biology Teacher, 69(4), 219-225.

Students cultivated C. elegans, measured C. elegans population over time to gain deeper understanding of Darwin’s premises about populations, such as natural selection.

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