Multiphoton Fluorescence Excitation Microscopy
Multiphoton microscopy is used to generate high resolution optical sections to image fluorescent signals, usually in live specimens.
This is a C. elegans
embryo in the first mitosis stage (anterior at the left and posterior at the right). Microtubules (MTs) are labeled using GFP.
MPFE Compared to Laser Scanning Confocal Microscopy
By using multi-photon for fluorophore excitation:
(Enable “selective excitation”, 31 KB, movie length: 1′ 7″)
(Enable “deeper sectioning & better specimen viability”, 1 MB, movie length: 48″)
Random Worm Term
Physical and functional unit of heredity which carries information from one generation to the next. In molecular terms, it is the entire DNA sequence – including exons, introns, and noncoding transcription-control regions – necessary for production of a functional protein or RNA.
sequence – including exons, introns, and noncoding transcription-control regions – necessary for production of a functional protein or RNA.
