summary: multiphoton microscopy is used to generate high resolution optical sections to image fluorescent signals, usually in live specimens.

A Multiphoton(MPFE)C. elegans image

This is a C. elegans embryo^G in the first mitosis stage (anterior at the left and posterior at the right). Microtubules (MTs)^G are labeled using GFP^G.

Bioclips: Advantage of MPFE compared to lscm

By using multi-photon to excite^G fluorophores^G

(Enable "selective excitation", 31 KB, movie length: 1′ 7")

(Enable "deeper sectioning & better specimen^G viability", 1 MB, movie length: 48")

Glossary:

Embryo: the early developmental stage of an organism (C. elegans in this case) after fertilization and before hatching.

Excite (fluorophore excitation): the act or process of bringing a fluorophore to its excitated state using light or laser sources.

GFP (Green Fluorescent Protein): a naturally occurring fluorescent protein synthesized by the jellyfish Aequora Victoria.

Fluorophore: a molecule that can be exited by the absorption of a photon and can decay to the ground state by emitting a longer wavelength fluorescent photon.

Microtubule (MT): long, cylindrical polymer composed of the protein tubulin. It is one of the three major classes of filaments in the cytoskeleton.

Specimen: any animal or plant, or any part, product, egg, seed or root
of any animal or plant. For example,C. elegans embryos can be specimens
that one can look at with light microscopy.