summary: multiphoton microscopy
is used to generate high resolution optical sections to image fluorescent
signals, usually in live specimens.
A Multiphoton(MPFE)C. elegans image
This is a C. elegans embryoG
in the first mitosis stage (anterior at the left and posterior at the
right). Microtubules (MTs)G are labeled
Bioclips: Advantage of MPFE compared to lscm
By using multi-photon to exciteG
(Enable "selective excitation", 31 KB, movie length: 1′ 7")
(Enable "deeper sectioning & better specimenG
viability", 1 MB, movie length: 48")
Embryo: the early developmental
stage of an organism (C. elegans in this case) after fertilization
and before hatching.
excitation): the act or process of bringing a fluorophore to its excitated
state using light or laser sources.
GFP (Green Fluorescent Protein):
a naturally occurring fluorescent protein synthesized by the jellyfish
a molecule that can be exited by the absorption of a photon and can
decay to the ground state by emitting a longer wavelength fluorescent
Microtubule (MT): long,
cylindrical polymer composed of the protein tubulin. It is one of the
three major classes of filaments in the cytoskeleton.
animal or plant, or any part, product, egg, seed or root
of any animal or plant. For example,C. elegans embryos can
that one can look at with light microscopy.