summary: multiphoton microscopy is used to generate high resolution optical sections to image fluorescent signals, usually in live specimens.
A Multiphoton(MPFE)C. elegans image
This is a C. elegans embryoG in the first mitosis stage (anterior at the left and posterior at the right). Microtubules (MTs)G are labeled using GFPG.
Bioclips: Advantage of MPFE compared to lscm
By using multi-photon to exciteG fluorophoresG
Embryo: the early developmental stage of an organism (C. elegans in this case) after fertilization and before hatching.
Excite (fluorophore excitation): the act or process of bringing a fluorophore to its excitated state using light or laser sources.
GFP (Green Fluorescent Protein): a naturally occurring fluorescent protein synthesized by the jellyfish Aequora Victoria.
Fluorophore: a molecule that can be exited by the absorption of a photon and can decay to the ground state by emitting a longer wavelength fluorescent photon.
Microtubule (MT): long, cylindrical polymer composed of the protein tubulin. It is one of the three major classes of filaments in the cytoskeleton.
animal or plant, or any part, product, egg, seed or root