Mutant Phenotype Analysis

Dominika Bienkowska

What is a wild type or a mutant? How do wild type and mutant develop similarly or differently? This module includes movies of wild type and 5 mutant C. elegans early embryonic development. These movies are in DIC (Differential Interference Contrast) format with magnification about 100X.

Early embryonic development of C. elegans from 1-cell zygote to about 8-cells stage was shown clearly. Background information regarding imaging and characterization of these movies is also provided. The materials can be used for various instructional purposes. For instance, it can be used to show how wild type and mutant develop differently, for students to characterize differences or similarities among development of wild type and mutants, or even to look up the mutants’ genetic information for more in-depth scientific inquiry.

Credit: The module was developed by Dominika Bienkowska during her working as an undergraduate researcher with the Laboratory for Optical and Computational Instrumentation.

Instructions: To use, simply unzip the file and open index.html with your web browser. You will need the QuickTime video player.

Major questions presented:

  • What is Wild Type and what is a Mutant?
  • How can you characterize Wild Type and Mutants?
  • Symmetric cell division: is it normal?
MutantPhenotypeAnalysis.zip147.76 MB