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Notes from the researcher:

• The researcher (Dr. Kevin O’Connell) who provided these data proposed that the defect in the protein level is in a specific kinase.
• The 3D structure for these amino acid sequences is unknown.
• The amino acid sequence of a protein consists of various numbers of amino acids. For example, Arg stands for Arginine, Pro means proline. You can find the conversion from one amino acid to its Letter symbol in any molecular biology textbook, such as Benjamin Lewin’s Genes series.

Thoughts:

• Some interesting questions might be raised while combining information from the “Genome Info” page and this page, such as the length of amino acid sequence and the number of codons in genome sequence. See whether you can have some other insights.
• By combining the information here with the “Genome Info” page, you can also compare the DNA codon to the amino acid sequence, some interesting relationship might be found.
• Tools for sequences comparison are provided down in the bottom of this page. For each program, you can simply copy and paste the sequence of your interest to any of the programs and see how sequences differ from each other. See whether you can gain some insight on the difference.

Movie Subject	Length of this Kinase (amino acids)		Amino acid sequences
WT	706		MSGGKSGSRL SAYSHLKEIG KGGFGVVYAA QRENGEKVAI KRIDKKVPKN RVWTEIQTMK ELKKSKYVVE FYEDFVEDGY TYIVMELCEG GSLQAYVREH GALDDATAVH VLRQLISAVS FMHRVNVIHR DLSAGNVFIK DSKKKKMTVK LGDFGLATTL GRGETTCTIV GTPGFIAPQV YDQEYTQSAD VYSLGAVLYT MLTARNPPPK GLPPTCGMSP NAARLVEQMM DTDAKKRIPL TQIVLSEFMY ENTNENAVIF SREHSRDGRR QRSREPVRSS RDDRSRDGRA LIRSSSQPAH SGRAPLSNRP IHDRMPSTSS RGFDSERGRE RDRDSGRGTV PPSREDRNRS QLWPIRMDRL EGQRVCTAGG RYIVELDTRC RFEVAAQGNF VKRILIVEVD EMVQTVYVHR IPDRTVRGRN GEEELITLTN NPFVYTSYSQ MPKEVQNDYM RLQKMVAVTI SGRVAKVTFR RPSQFPDAQA QLMENGDLRI KLPRSVIVRK MDNGEIFNCI DGIATQKQAV SGITLTKVNE VYKYLIRFEQ CLNGMDRGMV CFPIVFSAGT NMVGSSPSSL MPSGSSQTSR FPFSNLSNNQ PSLVPHSAPF LTKPTSSQRA SSANVQRRVS TDENSSPSVA PSKYKIKIDP TTQKVRSIQA TDGRVLRCST SKADQFIFTD PAIRPDDQRF MRTDRVPDRA SEMLHTLCER MRKLHQ
Mutant 1	706		MSGGKSGSRL SAYSHLKEIG KGGFGVVYAA QRENGEKVAI KRIDKKVPKN RVWTEIQTMK ELKKSKYVVE FYEDFVEDGY TYIVMELCEG GSLQAYVREH GALDDATAVH VLRQLISAVS FMHRVNVIHR DLSAGNVFIK DSKKKKMTVK LGDFGLATTL GRGETTCTIV GTPGFIAPQV YDQEYTQSAD VYSLGAVLYT MLTARNPPPK GLPPTCGMSP NAARLVEQMM DTDAKKRIPL TQIVLSEFMY ENTNENAVIF SREHSRDGRR QRSREPVRSS RDDRSRDGRA LIRSSSQPAH SGRAPLSNRP IHDRMPSTSS RGFDSERGRE RDRDSGRGTV PPSREDRNRS QLWPIRMDRL EGQRVCTAGG QYIVELDTRC RFEVAAQGNF VKRILIVEVD EMVQTVYVHR IPDRTVRGRN GEEELITLTN NPFVYTSYSQ MPKEVQNDYM RLQKMVAVTI SGRVAKVTFR RPSQFPDAQA QLMENGDLRI KLPRSVIVRK MDNGEIFNCI DGIATQKQAV SGITLTKVNE VYKYLIRFEQ CLNGMDRGMV CFPIVFSAGT NMVGSSPSSL MPSGSSQTSR FPFSNLSNNQ PSLVPHSAPF LTKPTSSQRA SSANVQRRVS TDENSSPSVA PSKYKIKIDP TTQKVRSIQA TDGRVLRCST SKADQFIFTD PAIRPDDQRF MRTDRVPDRA SEMLHTLCER MRKLHQ
Mutant 2	706		MSGGKSGSRL SAYSHLKEIG KGGFGVVYAA QRENGEKVAI KRIDKKVPKN RVWTEIQTMK ELKKSKYVVE FYEDFVEDGY TYIVMELCEG GSLQAYVREH GALDDATAVH VLRQLISAVS FMHRVNVIHR DLSAGNVFIK DSKKKKMTVK LGDFGLATTL GRGETTCTIV GTPGFIAPQV YDQEYTQSAD VYSLGAVLYT MLTARNPPPK GLPPTCGMSP NAARLVEQMM DTDAKKRIPL TQIVLSEFMY ENTNENAVIF SREHSRDGRR QRSREPVRSS RDDRSRDGRA LIRSSSQPAH SGRAPLSNRP IHDRMPSTSS RGFDSERGRE RDRDSGRGTV PPSREDRNRS QLWPIRMDRL EGQRVCTAGG RYIVELDTRC RFEVAAQGNF VKRILIVEVD EMVQTVYVHR IPDRTVRGRN GEEELITLTN NLFVYTSYSQ MPKEVQNDYM RLQKMVAVTI SGRVAKVTFR RPSQFPDAQA QLMENGDLRI KLPRSVIVRK MDNGEIFNCI DGIATQKQAV SGITLTKVNE VYKYLIRFEQ CLNGMDRGMV CFPIVFSAGT NMVGSSPSSL MPSGSSQTSR FPFSNLSNNQ PSLVPHSAPF LTKPTSSQRA SSANVQRRVS TDENSSPSVA PSKYKIKIDP TTQKVRSIQA TDGRVLRCST SKADQFIFTD PAIRPDDQRF MRTDRVPDRA SEMLHTLCER MRKLHQ
Mutant 3	615		MSGGKSGSRL SAYSHLKEIG KGGFGVVYAA QRENGEKVAI KRIDKKVPKN RVWTEIQTMK ELKKSKYVVE FYEDFVEDGY TYIVMELCEG GSLQAYVREH GALDDATAVH VLRQLISAVS FMHRVNVIHR DLSAGNVFIK DSKKKKMTVK LGDFGLATTL GRGETTCTIV GTPGFIAPQV YDQEYTQSAD VYSLGAVLYT MLTARNPPPK GLPPTCGMSP NAARLVEQMM DTDAKKRIPL TQIVLSEFMY ENTNENAVIF SREHSRDGRR QRSREPVRSS RDDRSRDGRA LIRSSSQPAH SGRAPLSNRP IHDRMPSTSS RGFDSERGRE RDRDSGRGTV PPSREDRNRS QLWPIRMDRL EGQRVCTAGG RYIVELDTRC RFEVAAQGNF VKRILIVEVD EMVQTVYVHR IPDRTVRGRN GEEELITLTN NPFVYTSYSQ MPKEVQNDYM RLQKMVAVTI SGRVAKVTFR RPSQFPDAQA QLMENGDLRI KLPRSVIVRK MDNGEIFNCI DGIATQKQAV SGITLTKVNE VYKYLIRFEQ CLNGMDRGMV CFPIVFSAGT NMVGSSPSSL MPSGSSQTSR FPFSNLSNNQ PSLVPHSAPF LTKPTSSQRA SSANV
Mutant 4	684		MSGGKSGSRL SAYSHLKEIG KGGFGVVYAA QRENGEKVAI KRIDKKVPKN RVWTEIQTMK ELKKSKYVVE FYEDFVEDGY TYIVMELCEG GSLQAYVREH GALDDATAVH VLRQLISAVS FMHRVNVIHR DLSAGNVFIK DSKKKKMTVK LGDFGLATTL GRGETTCTIV GTPGFIAPQV YDQEYTQSAD VYSLGAVLYT MLTARNPPPK GLPPTCGMSP NAARLVEQMM DTDAKKRIPL TQIVLSEFMY ENTNENAVIF SREHSRDGRR QRSREPVRSS RDDRSRDGRA LIRSSSQPAH SGRAPLSNRP IHDRMPSTSS RGFDSERGRE RDRDSGRGTV PPSREDRNRS QLWPIRMDRL EGQRVCTAGG RYIVELDTRC RFEVAAQGNF VKRILIVEVD EMVQTVYVHR IPDRTVRGRN GEEELITLTN NPFVYTSYSQ MPKEVQNDYM RLQKMVAVTI SGRVAKVTFR RPSQFPDAQA QLMENGDLRI KLPRSVIVRK MDNGEIFNCI DGIATQKQAV SGITLTKVNE VYKYLIRFEQ CLNGMDRGMV CFPIVFSAGT NMVGSSPSSL MPSGSSQTSR FPFSNLSNNQ PSLVPHSAPF LTKPTSSQRA SSANVQRRVS TDENSSPSVA PSKYKIKIDP TTQKVRSIQA TDGRVLRCST SKADQFIFTD PAIRPDDQRF MRTD

* Wondering where the wild type sequence is from? Try WormBase. Brief instructions on how to search can be found here.