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Research Protocols (50)

Ballistic Transformation (Schedl Lab)

http://genetics.wustl.edu/tslab/protocols/ballistic-transformation/

Research Protocols

Fermentor worm chow

http://www.k-state.edu/hermanlab/protocols/fermentor.htm

Clicked 195 times. Last clicked 05/11/2013 – 04:43.

Research Protocols

Lethal phase determination

http://medicine.yale.edu/labs/koelle

Clicked 332 times. Last clicked 12/04/2014 – 17:08.

Research Protocols

Mircroinjecting worms

http://info.med.yale.edu/mbb/koelle/protocols/protocol_microinjecting w.html

Clicked 586 times. Last clicked 05/14/2013 – 00:15.

Research Protocols

Worm Breeder’s Gazette

http://www.wormbook.org/wbg/

Discusses current techniques for researching C. elegans. Recently revived; full archives are also available on the site.

The Worm Breeder’s Gazette is an informal, non-refereed, biannual newsletter for the interchange of ideas and information related to C. elegans and other nematodes. The WBG provides an opportunity for researchers to announce experimental results not yet published, correct errors in or supplement published work, and describe methods and techniques that might be useful to the whole community.

Clicked 376 times. Last clicked 06/11/2017 – 02:39.

Research Protocols

Differential Interference Contrast (DIC) Microscopy

http://micro.magnet.fsu.edu/primer/techniques/dic/dichome.html

By Molecular Expressions Website Information: with information such as brief overview of DIC, basic concepts of DIC, comparison of DIC with phase contrast etc.

Clicked 364 times. Last clicked 05/20/2017 – 18:41.

Microscopy

Laser scanning confocal microscopy

http://micro.magnet.fsu.edu/primer/techniques/confocal/index.html

By Molecular Expressions Website Information : this contains detailed information such as basic concepts of LSCM, imaging mode, specimen preparation etc.

Clicked 313 times. Last clicked 07/12/2017 – 17:31.

Microscopy

Molecular Expressions

http://micro.magnet.fsu.edu/index.html

“This website is brought to you by the Optical Microscopy Division of the National High Magnetic Field Laboratory, a joint venture of The Florida State University, the University of Florida, and the Los Alamos National Laboratory.” This is a comprehensive web site for optical microscopy.

Clicked 294 times. Last clicked 06/11/2017 – 02:39.

Microscopy

Molecular and Biochemical Techniques (7)

Genomic DNA prep

http://www.k-state.edu/hermanlab/protocols/genomic_dna_prep.htm

Clicked 298 times. Last clicked 05/14/2017 – 17:09.

Molecular and Biochemical Techniques

PCR analysis of dead eggs from C. elegans

http://saturn.med.nyu.edu/research/mp/ronlab/protocols/DeadEggsPCR.html

Clicked 291 times. Last clicked 10/04/2016 – 16:02.

Molecular and Biochemical Techniques

Single worm PCR

http://thalamus.wustl.edu/nonetlab/ResourcesF/WormPCR.htm

Clicked 281 times. Last clicked 07/30/2017 – 14:44.

Molecular and Biochemical Techniques

Worm genomic southern blots

http://medicine.yale.edu/labs/koelle

Clicked 221 times. Last clicked 05/10/2013 – 06:49.

Molecular and Biochemical Techniques

Mutagenesis (7)

C. elegans gene knockout protocol

http://medicine.yale.edu/labs/koelle

Clicked 274 times. Last clicked 03/20/2015 – 13:07.

Mutagenesis

DpnI mediated site-directed mutagenesis

http://thalamus.wustl.edu/nonetlab/ResourcesF/dpnmut.html

Clicked 371 times. Last clicked 05/07/2016 – 03:34.

Mutagenesis

EMS mutagenesis

http://medicine.yale.edu/labs/koelle

Clicked 242 times. Last clicked 12/05/2015 – 15:52.

Mutagenesis

EMS mutagenesis (Kansas State University)

http://www.k-state.edu/hermanlab/protocols/ems_mutagenesis.htm

Clicked 377 times. Last clicked 06/11/2017 – 02:35.

Mutagenesis

Making males by heat shock

http://medicine.yale.edu/labs/koelle

Clicked 258 times. Last clicked 03/13/2015 – 11:42.

Mutagenesis

RNAi (4)

dsRNA preparation for RNAi by injection in C. elegans (PDF)

http://labs.bio.unc.edu/Goldstein/dsRNAprepGoldsteinLab.pdf

Clicked 246 times. Last clicked 05/14/2013 – 00:13.

RNAi

Making RNAi primers

http://elegans.bcgsc.bc.ca/oligos.shtml

Clicked 313 times. Last clicked 02/25/2015 – 02:50.

RNAi

RNAi experiments

http://avery.rutgers.edu/WSSP/StudentScholars/project/protocols/RNAi.html

Clicked 356 times. Last clicked 10/15/2016 – 00:20.

RNAi

Silencing Genomes

http://www.silencinggenomes.org/

Clicked 393 times. Last clicked 06/11/2017 – 02:36.

RNAi

Double dye filling

http://www.k-state.edu/hermanlab/protocols/double_dye_fill.htm

Clicked 220 times. Last clicked 02/25/2015 – 02:54.

Staining

EdU labeling and staining (Schedl Lab)

http://genetics.wustl.edu/tslab/protocols/edu-labeling-and-staining/

Staining

Fixation

http://thalamus.wustl.edu/nonetlab/ResourcesF/staining.htm

Clicked 279 times. Last clicked 05/05/2016 – 09:17.

Staining

MMS integration

http://www.k-state.edu/hermanlab/protocols/mms_integration.htm

Clicked 230 times. Last clicked 12/05/2016 – 15:06.

Staining

Nematode dye filling

http://www.k-state.edu/hermanlab/protocols/nematode_dye_filling.htm

Clicked 247 times. Last clicked 04/29/2017 – 07:32.

Staining

Standard Osmium/Glutheraldehyde Fixation

http://thalamus.wustl.edu/nonetlab/ResourcesF/EMprotocol.htm

Clicked 253 times. Last clicked 05/14/2013 – 00:15.

Staining

Worm Culture (15)

“Chunking” worms – easy strain maintenance

http://home.sandiego.edu/~cloer/loerlab/chunking.html

Clicked 378 times. Last clicked 05/15/2017 – 01:02.

Worm Culture

Bacteria for worm food

http://home.sandiego.edu/~cloer/loerlab/wormfood.html

Clicked 311 times. Last clicked 01/21/2017 – 23:35.

Worm Culture

Cloning form low melt agrose

http://thalamus.wustl.edu/nonetlab/ResourcesF/clone.html

Clicked 401 times. Last clicked 06/14/2016 – 22:06.

Worm Culture

Cool growth competent E. coli

http://thalamus.wustl.edu/nonetlab/ResourcesF/comps.html

Clicked 276 times. Last clicked 04/06/2016 – 04:41.

Worm Culture

Culturing worms

http://medicine.yale.edu/labs/koelle

Clicked 273 times. Last clicked 07/27/2017 – 00:37.

Worm Culture

ES cell culture

http://saturn.med.nyu.edu/research/mp/ronlab/protocols/ES_cell_culture.html

Clicked 1873 times. Last clicked 05/08/2017 – 02:23.

Worm Culture

Freezing worms

http://medicine.yale.edu/labs/koelle

Clicked 254 times. Last clicked 01/01/2014 – 23:18.

Worm Culture

Growing worms in liquid

http://www.k-state.edu/hermanlab/protocols/growing_worms_in_liquid.htm

Clicked 369 times. Last clicked 05/12/2017 – 03:32.

Worm Culture

Liquid culture of worms

http://medicine.yale.edu/labs/koelle

Clicked 238 times. Last clicked 05/10/2013 – 00:14.

Worm Culture

Liquid culture of worms in with fermentor

http://avery.rutgers.edu/WSSP/StudentScholars/project/protocols/maintenance.html

Clicked 454 times. Last clicked 08/09/2017 – 10:02.

Worm Culture

N2 Development time at different temperatures

http://medicine.yale.edu/labs/koelle

Clicked 229 times. Last clicked 05/10/2013 – 00:14.

Worm Culture

Preparation of petri plates (PDF)

http://www.childrenshospital.org/silverman/protocols/elegans/preparation.pdf

Clicked 116 times. Last clicked 06/11/2017 – 02:34.

Worm Culture

Preparation of synchronized cultures

http://www.childrenshospital.org/silverman/protocols/elegans/synchronized_cultures.pdf

Clicked 115 times. Last clicked 06/11/2017 – 02:35.

Worm Culture

Quick bleach cleaning of small numbers of worms

http://home.sandiego.edu/~cloer/loerlab/bleachclean.html

Clicked 392 times. Last clicked 04/13/2017 – 12:03.

Worm Culture

Response to food assay

http://medicine.yale.edu/labs/koelle

Clicked 225 times. Last clicked 08/17/2015 – 11:59.

Worm Culture