Useful methods for producing high quality cultures and movies for C. elegans teaching labs at large (and small) institutions.
For this experiment, you will be given worms with a dominant mutation in the
rol-6 gene. You will also be given some wild
type worms so that you can observe the normal movement pattern.
You will then use RNAi (also known as RNA interference) to knockdown
expression of the mutant rol-6 allele
. What do you expect will happen to the worms
after the RNAi treatment?
What is a wild type or a mutant
? How do wild type and mutant develop similarly or differently? This module includes movies of wild type and 5 mutant C. elegans
‘ early embryonic development. These movies are in DIC (Differential Interference Contrast) format with magnification about 100X.
Adult male, protruding
Same as previous, with different focal plane highlighted
At left: L3 male with slightly less developed tail. The animal on the right is L4 male with more developed tail.
Male animal: Fan, hook, half of the body
Identification of dumpy worms; design of primers to amplify dpy-5 gene. Preparation of single worms for PCR, PCR reactions, gel electrophoresis.
Staining transgenic worms for ß-galactosidase. Also includes a list of supplies and solutions required for ß-galactosidase staining lab.
This PowerPoint lecture describes C. elegans early embryo
development. It also includes detailed description about C. elegans gastrulation.
The embryonic cell lineage of C. elegans
from zygote
to hatched larva
provides an excellent tool for learning fundamental principals in developmental biology.
Lab for observing bacterial-mediated feeding RNAi. Also includes solutions for use with bacterial-mediated feeding RNAi (as well as other labs).